19 U.S. AI Startups That Have Raised $100M+ in 2025 So Far
Despite global economic headwinds, 2025 is proving to be another blockbuster year for U.S. artificial intelligence startups. As of April, 19 AI companies have already secured funding rounds of $100 million or more — a continuation of the momentum seen in 2024.
From generative AI platforms to AI-driven healthcare and defense solutions, venture capital firms are aggressively backing innovation. Here’s a breakdown of the top startups that have hit the $100M mark this year:
April Highlights
- SandboxAQ raised $450M Series E (valuation: $5.7B) with investors like Nvidia, Google, and Ray Dalio.
- Runway secured $308M Series D for AI media tools (valuation: $3B).
March Milestones
- OpenAI shocked the market with a $40B round, boosting its valuation to a staggering $300B.
- Celestial AI raised $250M Series C (valuation: $2.5B) for infrastructure.
- Shield AI closed a $240M Series F in defense tech (valuation: $5.3B).
- Anthropic landed a $3.5B Series E, the year’s biggest (valuation: $61.5B).
- Other notables include:
- Turing ($111M Series E)
- Nexthop AI ($110M Series A)
- Insilico Medicine ($110M Series E)
- Lila Sciences ($200M seed)
- Reflection.AI ($130M Series A)
February Movers
- Together AI pulled in $305M Series B (valuation: $3.3B).
- Lambda closed a $480M Series D.
- Abridge raised $250M Series D (valuation: $2.75B).
- Eudia and EnCharge AI raised $105M and $100M, respectively.
- Harvey, focused on legal tech, raised $300M Series D (valuation: $3B).
January Starters
- ElevenLabs kicked off 2025 with a $180M Series C (valuation: $3B+).
- Hippocratic AI followed with a $141M Series B (valuation: $1.6B+).
This surge reflects growing confidence in AI’s ability to disrupt multiple sectors, including healthcare, defense, software development, and media.
With many months still ahead, 2025 could surpass last year’s record of 49 megarounds. Investors are clearly betting big on the AI boom — and the race for innovation is just heating up.
